ISOLATION AND BIOCHEMICALCHARACTERIZATION OF SALMONELLA & E.COLI FROM BOVINE MILK COLLECTED FROM SALE SHOPS, GOVERNAMENTAL & PRIVATE DAIRY FARMS AT QUETTA PAKISTAN
A total of 100 raw milk samples of bovine were collected in sterile screw capped tubes(each 5ml) from Government Dairy Farm(45 samples) , Private Dairy Farms (45 samples) and Private Milk Shops(10 samples) from different locations in Quetta city to isolate milk borne microbial pathogens like Salmonella and E Coli to estimate their share in the environmental pollution. All these samples were subjected to grow on Salmonella Shigella agar (SS Agar) and Xylose Lysine Deoxycholate (XLD) agar. The isolated colonies were further identified biochemically through triple sugar iron test (TSI) and Gram’s staining. From Government Dairy Farm out of forty five (45) raw milk samples six (6) samples (13%) were found positive for E Coli whereas no salmonella (0%) colony was detected. While from Private Dairy Farms out of Forty Five (45) samples ten(10) were positive for E Coli(22%) and only one(1) sample was positive(2%) for Salmonella. From private milk shops out of ten (10) samples four (4) were positive for (40%) E coli and three (3) for salmonella (30%). It is concluded from the above study that there is 4% Salmonella and 20% E.coli contamination in raw milk samples being supplied to the people in Quetta city. The high proportion of contamination (30%) of Salmonella and (40%) of E.coli was detected from Private Milk Shops due to Exposure of milk to open Environment where these both pathogens are present. which is a serious public health issue and need to create a public awareness to use treated (Pasteurized) milk and proper disposal of the waste from dairy farms as well as shops and as well as to avoid exposure of milk to open contaminated Environment of the city.
Salmonella; E.coli; Milk born diseases; Environment