Green pre-treatment method and Lignin extraction from lignocellulose biomass for enhanced biofuel production

Mohammad Siddique Nasar, Suhail Ahmed Soomro, Shaheen Aziz, Ehsanullah Khan Kakar, Ali Nawaz Mengal, Luqman Khan, Wisdom chukwuemeke Ulakpa, Kamran Khan, Namat ullah


The need for a flexible strategy to meet rising global energy demands has led many academics to concentrate on renewable biofuel made from sustainable resources, particularly lignocellulose feedstock.   After cellulose, lignin is the most prevalent natural renewable polymer. From lignocellulosic material, it can be extracted. Due to the demand for various pure raw materials derived from biomass resources, interest in lignin has recently increased. It has been determined that certain resources, such as walnut shell (WNS), almond shell (AS), neem tree bark (NTB), and babool tree bark (BTB), have additional uses. We cover the removal of lignin from biomass in this essay. In a particle technology lab, sieve studies were carried out utilising a magnetic sieve shaker and a first sieve with a size of 1000 m. The electrical muffle furnace underwent the proximate studies, as well as the final analyses for C, H, N, and S. Lignin yield was obtained using a straightforward extraction process based on NaOH and H2SO4. Effective valorization of lignocellulose biomass into high-value chemicals and biofuels utilizing sustainable, environmentally friendly technology. This paper's major goal is to produce and extract lignin from biomass.  We present and discuss lignin biomass pre-treatment techniques. The ideal process variables that enhance lignin breakdown are examined for green pre-treatment techniques.  The purity of the resulting lignin samples was determined using FT-IR (Fourier Transform Infrared Spectroscopy),EDS,SEM were done. The results show that an extraction process lasting 5 hours, 4 hours, and 3 hours at 100 degrees centigrade, 130 degrees centigrade, and 160 degrees centigrade, using NaOH and H2SO4 for liquor and biomass to liquor ratio of 1:20, is effective.


Pretreatment, Lignocellulosic Green technology, Lignin, pyrolysis,sustainable, Extraction

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J. Keskiväli, P. Wrigstedt, K. Lagerblom, and T. Repo, “One-step Pd/C and Eu(OTf)3 catalyzed hydrodeoxygenation of branched C11 and C12 biomass-based furans to the corresponding alkanes,” Appl. Catal. A Gen., vol. 534, pp. 40–45, 2017, DOI: 10.1016/j.apcata.2017.01.017.

P. Ibarra-Gonzalez and B. G. Rong, “Systematic Synthesis and Evaluation of Thermochemical Conversion Processes for Lignocellulosic Biofuels Production: Total Process Evaluation and Integration,” Ind. Eng. Chem. Res., vol. 57, no. 30, pp. 9925–9942, 2018, doi: 10.1021/acs.iecr.7b05382.

Rezania, S., Oryani, B., Cho, J., Talaiekhozani, A., Sabbagh, F., Hashemi, B., Rupani, P.F. and Mohammadi, A.A., 2020. Different pretreatment technologies of lignocellulosic biomass for bioethanol production: an overview. Energy, 199, p.117457.

Raud, M., Kikas, T., Sippula, O. and Shurpali, N.J., 2019. Potentials and challenges in lignocellulosic biofuel production technology. Renewable and Sustainable Energy Reviews, 111, pp.44-56.

P. Nimmanterdwong, B. Chalermsinsuwan, and P. Piumsomboon, “Prediction of lignocellulosic biomass structural components from ultimate/proximate analysis,” Energy, vol. 222, p. 119945, 2021, doi: 10.1016/

Tong, W., Chu, Q., Li, J., Xie, X., Wang, J., Jin, Y., Wu, S., Hu, J. and Song, K., 2022. Insight into understanding sequential two-stage pretreatment on modifying lignin physiochemical properties and improving holistic utilization of renewable lignocellulose biomass. Renewable Energy, 187, pp.123-134.

N. Mt, P. D. F. Pack, B. Laborat, L. Feedst, and L. Feedst, “A national laboratory of the National Renewable Energy Laboratory Innovation for Our Energy Future Determination ... Determination of Structural Carbohydrates and Lignin in Biomass Laboratory Analytical Procedure ( LAP ).”

Li, Z., Otsuki, A.L. & Mascal, M. 2018. Production of cellulosic

gasoline: Via levulinic ester self-condensation. Green

Chemistry 20(16): 3804–3808.

M. Wu, J. Peng, Y. Dong, J. Pang, and X. Zhang, “Extraction and oxypropylation of lignin by an efficient and mild integration process from agricultural waste,” Ind. Crops Prod., vol. 172, no. September, p. 114013, 2021, doi: 10.1016/j.indcrop.2021.114013.

A. Ma’Ruf, B. Pramudono, and N. Aryanti, “Optimization of lignin extraction from rice husk by alkaline hydrogen peroxide using response surface methodology,” Rasayan J. Chem., vol. 10, no. 2, pp. 407–414, 2017, doi: 10.7324/RJC.2017.1021667.

M. Siddique, S. Ahmed, S. Aziz, and F. Akhter, “An Overview of Recent Advances and Novel Synthetic Approaches for Lignocellulosic derived Biofuels,” J. Kejuruter., vol. 33, no. 2, pp. 165–173, 2021.

U. M. Ahmad et al., “Can lignin be transformed into agrochemicals? Recent advances in the agricultural applications of lignin,” Ind. Crops Prod., vol. 170, no. October 2020, 2021, doi: 10.1016/j.indcrop.2021.113646.

F. Mushtaq et al., “Fluidized bed heat exchange capacity of Alumina, coal-char and bio-char solids,” IOP Conf. Ser. Mater. Sci. Eng., vol. 414, no. 1, 2018, doi: 10.1088/1757-899X/414/1/012003.

Akhter,F., Soomro, S.A., Jamali, A. R., Chandio, Z. A., Siddique, M., Ahmed, M. (2021): “Rice husk ash as green and sustainable biomass waste for construction and renewable energy applications : a review,”

F. Akhter and M. Siddique, “Plant and Non-plant based Polymeric Coagulants for

Wastewater Treatment : Plant and Non-plant based Polymeric Coagulants for

Wastewater Treatment : A Review,” vol. 33, no. May, 2021, doi: 10.17576/jkukm-2021-



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