Evaluation of Seed Viability of Juniperus Excelsa (M. Bieb.) found in Balochistan by Tetrazolium Assay

Muhammad Hussain Khajjak, Agha Muhammad Raza, Muhammad Luqman, Asma Yousafzai, Sara Naudhani, Ghazala Muhammad Ali, Muhammad Saeed

Abstract


The present study was carried out to determine the seed viability of seeds of Juniperus excelsa by Tetrazolium (TZ) assay. The cones/berries of J. excelsa were collected from three locations in Balochistan. The preparation of the seeds for the biochemical assay was performed as recommended by the International Seed Testing Agency (ISTA) rules 2007. The seeds of J. excelsa were tested for viability in aqueous solution of 2,3,5-triphenyl tetrazolium chloride of three different concentrations. The staining pattern of the seeds differed with respect to the strength of the indicator. Only (1%) seeds from Ziarat, Zarghoon Ghar and Harboi provenances were found to develop a partial and light pink staining of the tissues in (0.5%) aqueous solution of TZ. The percentages of seeds that completely stained in (1%) aqueous solution of TZ were found (5%), (4%) and (5%) for Ziarat, Zarghoon Ghar and Harboi provenances respectively. The percentages of partially stained seeds in (1%) aqueous solution of TZ were found (6%), (5%), and (6%) for Ziarat, Zarghoon Ghar and Harboi provenances respectively. Finally the unstained seeds percentages for Ziarat, Zarghoon Ghar and Harboi provenances were found (89%), (91%) and (89%) respectively.

Keywords


Cupressaceae; Juniperus excelsa; seed viability; Tetrazolium chloride

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